绵羊线粒体基因变异与产羔数性状的关联分析

doi:10.11843/j.issn.0366-6964.2016.03.005

Abstract

Abstract:

The aim of the present study was to explore the effects of mtDNA on litter size in sheep.Genome sequencing and genotyping technologies were used for DNA variation analyses on mitochondrial coding genes in Dorper，Dorset and Suffolk sheep.In Dorper sheep，twenty-five mtDNA variations were detected，including 2 mutations in rRNA and tRNA genes，respectively，and 21 variations in polypeptide-coding genes(including 9 missense mutations and 12 synonymous mutations).For Dorset sheep，totally 20 variations，including 5 mutations in rRNA genes，1 mutation in tRNA genes，and 14 substitutions in polypeptide-coding genes(including 3 missense mutations and 11 synonymous mutations) were detected.In Suffolk sheep，there were 77 mutations in mtDNA coding genes，including 7 and 3 substitutions in rRNA and tRNA genes，and 67 mutations in polypeptide-coding genes which contained 7 missense mutations and 60 synonymous mutations.The mtDNA mutations presented poor associations with litter size in Dorper and Dorset sheep.However，in Suffolk sheep，14 mtDNA mutations were significantly associated with litter size(P＜0.05)，which both of the maximal values for the effects of the single mutation(T7759C) and haplotype(ATTCATTAAACTTT) were 0.22 per parity.The mtDNA had poor effects on litter size in Dorper and Dorset sheep.Mitochondrial DNA variations were significantly associated with litter size in Suffolk sheep.

CLC Number:

S826
S813.3

CHEN Xiao-yong，SUN Hong-xin，YUAN Ming，KANG Zhen-jiang，DUN Wei-tao，ZHAO Xing-bo. Association Analyses between Mitochondrial Coding Gene Variations and Sheep Litter Size[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 449-456.

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References

［1］张胜泉.鸡线粒体基因组遗传变异与屠宰性状的相关分析［D］.北京：中国农业大学，2013.
ZHANG S Q.Association of chicken mitochondrial genome variations and carcass traits［D］.Beijing：China Agricultural University，2013.(in Chinese)
［2］BELL B R，MCDANIELO B T，ROBISON O W.Effects of cytoplasmic inheritance on production traits of dairy cattle［J］.J Dairy Sci，1985，68(8)：2038-2051.
［3］QIN Y H，CHEN S Y，LAI S J.Polymorphisms of mitochondrial ATPase 86 genes and association with milk production traits in Holstein cows［J］.Anim Biotechnol，2012，23(3)：204-212.
［4］ZHANG B，CHEN H，HUA L，et al.Novel SNPs of the mtDNA ND5 gene and their associations with several growth traits in the Nanyang cattle breed［J］.Biochem Genet，2008，46(5-6)：362-368.
［5］MANNEN H，KOJIMA T，OYAMA K，et al.Effect of mitochondrial DNA variation on carcass traits of Japanese Black cattle［J］.J Anim Sci，1998，76(1)：36-41.
［6］MANNEN H，MORIMOTO M L，OYAMAT K，et al.Identification of mitochondrial DNA substitutions related to meat quality in Japanese Black cattle［J］.J Anim Sci，2003，81(1)：68-73.
［7］赵兴波，吴常信，李宁.太湖猪高繁殖性能的核外基因效应分析［J］.中国畜牧杂志，1999，35(3)：52-54.
ZHAO X B，WU C X，LI N.Analysis of extracellular genetic effect on reproductive performance in Taihu swine［J］.Chinese Journal of Animal Science，1999，35(3)：52-54.(in Chinese)
［8］FERNÁNDEZ A I，ALVES E，FERNÁNDEZ A，et al.Mitochondrial genome polymorphisms associated with longissimus muscle composition in Iberian pigs［J］.J Anim Sci，2008，86(6)：1283-1290.
［9］HIENDLEDER S.Molecular characterization of the sheep mitochondrial genome［J］.J Anim Breed Genet，1996，113(1-6)：293-302.
［10］REICHER S，SEROUSSI E，WELLER J I，et al.Ovine mitochondrial DNA sequence variation and its association with production and reproduction traits within an Afec-Assaf flock［J］.J Anim Sci，2012，90(7)：2084-2091.
［11］REED P D，VAN VLECK L D.Lack of evidence of cytoplasmic inheritance in milk production traits of dairy cattle［J］.J Dairy Sci，1987，70(4)：837-841.
［12］VAN VLECK L D，HANFORD K J，SNOWDER G D.Lack of evidence for cytoplasmic effects for four traits of Polypay sheep［J］.J Anim Sci，2005，83(3)：552-556.
［13］郭建平，刘永斌，王瑞.BMPR-IB基因突变与绵羊繁殖性能的研究进展［J］.当代畜禽养殖业，2008(8)：9-11.
GUO J P，LIU Y B，WANG R.Research progress in BMPR-IB gene mutation and ovine reproduction productivity［J］.Modern Animal Husbandry，2008(8)：9-11.(in Chinese)
［14］CAMPBELL B K，KENDALL N R，BAIRD D T.Effect of direct ovarian infusion of bone morphogenetic protein 6(BMP6) on ovarian function in sheep［J］.Biol Reprod，2009，81(5)：1016-1023.
［15］CHU M X，MU Y L，FANG L，et al.Prolactin receptor as a candidate gene for prolificacy of small tail han sheep［J］.Anim Biotechnol，2007，18(1)：65-73.

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Association Analyses between Mitochondrial Coding Gene Variations and Sheep Litter Size

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